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Minimize air exposure by using the smallest gauge needle practical (typically 2527 gauge for peptide withdrawal) and avoid injecting excess air into the vial when equalizing pressure

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These vesicles carry molecules such as miRNAs, mRNAs, and proteins, which participate in multiple aspects of folliculogenesis, including primordial follicle formation, oocyte development and selection, bidirectional granulosa-oocyte communication, steroid hormone regulation, and modulation of cumulus cell density, viability, proliferation, and apoptosis

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