(2) For adherent cells: wash 2 to 3 times with pre-cooled PBS at 4C, scrape off the cells with a cell scraper or treat the cells with EDTA solution so that the cells are no longer adhering very tightly to the wall, and blow down the cells with a pipette
Then, plates were washed with PBS, fixed with 4% paraformaldehyde, permeabilized by 0.2% Triton X-PBS solution, repeatedly washed by PBS, and stained with FITC-labeled phalloidin to bounds with the F-actin ring in cells for 1 h in darkness
Managing acne during TRT involves proper skincare, adjusting TRT dosage, topical treatments, oral medications, dermatologist consultation, lifestyle changes, patience, and consistency
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