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These changes can cause oxidative modifications to reactive thiols of structurally- or functionally-critical proteins, such as transcription factors 43 , signal transduction molecules 44 or metabolic enzymes 45 , and thereby evoke cellular defense mechanisms against oxidative damage 46

doi: 10.1080/00365521.2018.1553062

Handling and Aseptic Technique Proper handling is essential to maintain the integrity of BAC Water in research use: Withdrawals should be performed with sterile syringes or equipment The stopper and contact surfaces should be disinfected prior to each use Containers should be stored in accordance with laboratory guidelines and discarded if contamination is suspected While the solution is bacteriostatic, not bactericidal, adherence to aseptic technique is still required to minimize experimental variability and contamination risk

This suggests that the elevations were partially due to the presence of heavy metals, especially tin, tungsten, and antimony