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Notably, impairing -glutamylcysteine biosynthesis in the cytosol, but not in mitochondria, still allowed -glutamylcysteine to efficiently detoxify ROS

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The half-lives of unbound IGF-1, IGF-1 in a binary complex, and IGF-1 in a ternary complex are less than 10 minutes, 25 minutes, and more than 16 hours respectively meaning that IGF-1 activity is tightly regulated by its binding proteins

The V-rescale thermostat and Parrinello-Rahman barostat were used to maintain a constant 300 K temperature and 1 bar of pressure during the MD simulations following the initial energy minimization and system equilibration 37,38