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Cells were post-fixed for 1 h with 1% osmium tetroxide (Agar Scientific) in CACO buffer, dehydrated in a graded series of methanol and embedded in LR white resin

A 5mg/mL concentration allows measurements in 100mcg increments using the smallest markings on a standard 1mL insulin syringe (each 0.02mL or 2-unit increment = 100mcg)

However, a major shift occurred in November 2025 when the Trump administration negotiated MFN pricing deals with Novo Nordisk and Eli Lilly, reducing Medicare/Medicaid prices for Ozempic, Wegovy, Zepbound, and Mounjaro to approximately $245/month , with Medicare beneficiary co-pays of $50/month ( [17] ) ( [22] )