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Predicted microbial metabolites The relative abundance of 77 caecal bacterial metabolites was bioinformatically predicted using Melonnpan, based on the gene family (UniRef90) output of HUMAnN 3

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Cryo-EM data processing RELION-3.1.2 were used for all data processing to yield the polished particle stack that was subjected into cryoSPARC v4.4.1 for 3D reconstitution 28,29