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These include direct inhibition of inflammasome components, caspase-1 blockade, modulation of immune responses using anti-inflammatory agents, and targeted manipulation of gut microbiota and bile acid metabolism, which are increasingly recognized for their roles in liver homeostasis and injury response

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acute inflammatory conditions, myeloproliferative neoplasms, liver disease)16 or the presence of anti-intrinsic factor antibodies that can cause interference with the B12 assay, resulting in an artefactually high total B12 concentration.17 Active B12 (holotranscobalamin) Active B12 (holotranscobalamin) testing measures the biologically active fraction of B12 available to cells and is particularly useful for assessing potential deficiency in individuals with indeterminate total serum B12 concentrations