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Eighty female 8-week-old Balb/c mice were provided by Charles River, which were then housed under conditions of a light/dark cycle of 12 h, an ambient temperature of 25 2 C, and a humidity of 60 10%

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Greiner Bio-One GmbH, Baden-Wrttemberg, Germany), at a density of 0.8 10 4 cells/cm 2 and cultured in DMEM-F12 for three days