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Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail

Possible side effects of semaglutide include: nausea and vomiting constipation or diarrhea headache abdominal pain fatigue hypoglycemia Possible side effects of phentermine include: insomnia headache fast or irregular heartbeat dry mouth mood changes hypertension heart failure hallucinations reduced sex drive Additionally, people who take high doses of phentermine may experience withdrawal symptoms

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