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Cells were fixed with 4% paraformaldehyde for 30 min and washed with non-permeabilizing or permeabilizing PBS containing 0.025% Triton-X

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Vitamin C also plays a role in the immunity and improves the absorption of iron

Full contraindications list Your Lemon Bottle consent form should screen for all of the following contraindications: Pregnancy or breastfeeding Liver or kidney disease Autoimmune conditions (lupus, rheumatoid arthritis, scleroderma) Active skin infections or inflammation at the treatment site Blood clotting disorders or anticoagulant medication Allergy to bromelain, lecithin, riboflavin, or pineapple Diabetes (Type 1 or Type 2) Recent injectable treatments in the same area (past 4 weeks) History of keloid or hypertrophic scarring Thyroid conditions Cancer or undergoing chemotherapy/radiotherapy BMI over 30 (Lemon Bottle targets localised fat, not general weight loss) Risk categories explained Common side effects are part of the bodys normal inflammatory response to the treatment