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Dose and frequency depend on your B12 levels and underlying condition

J Histochem Cytochem (2004) 52:30110.10.1177/002215540405200301 24 TschopMWeyerCTataranniPADevanarayanVRavussinEHeimanML

Concomitantly, ROS can oxidatively activate Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) via methionine oxidation (e.g., Met281/282 oxidation), producing sustained kinase activity that phosphorylates mitochondrial Ca 2+ handling proteins such as the mitochondrial calcium uniporter (MCU) or its regulatory subunits

Never freeze reconstituted peptides, never shake the vial during mixing, and always use sterile draw technique to minimize headspace contamination between doses