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LC separation, which followed the previously reported procedure 28 , was performed using a normal-phase (hydrophilic interaction chromatography, HILIC) column (ZIC-pHILIC

With age, GSH levels decrease by up to 50% compared to young subjects, contributing to increased oxidative stress and chronic low-grade inflammation (inflammaging). In the anti-ageing field, GSH IV acts on three main levels: direct neutralisation of free radicals and protection of dermal collagen, modulation of inflammation through NF-B inhibition, and reduction of melanogenesis through tyrosinase inhibition with clinically observable effects on skin tone and luminosity. The most robust clinical evidence concerns neuroprotection (improvement of motor symptoms in Parkinson's disease) and protection from chemotherapy-induced neuropathy

6,7,8 The antioxidant effect of uric acid can be manifested in the inhibition of cell death to protect nerves as well as profile support of NO-mediated vasodilation

Ophthalmology 87 (1980):222-8