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The purified double-stranded cDNA was repaired by terminal repair, and a tail was added and sequenced

The high maximum daily defecations frequency group was characterized by older age, poorer kidney and heart function, a higher proportion of severe cases requiring intra-aortic balloon pumping or venoarterial extracorporeal membrane oxygenation, and more frequent use of antibiotics compared with the low maximum daily defecations frequency group (Supplemental Table 3)

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