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To measure respiration in sperm, Seahorse microplates were coated with 0.5 mg/mL concanavalin A (Sigma, C5275) in PBS for 1 hour before removing the coating and allowing the plate to dry overnight at room temperature as previously reported 135
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Due to the anti-inflammatory properties of cortisol, including the inhibition of IL-6 production (46), a relation between these parameters is very likely and may be supported by a significant correlation (r = 0.58) between IL-6 and cortisol in our study
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