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Light exposure accelerates degradation, making amber vials or foil wrapping advisable

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Sample buffer containing SDS, glycerol, Tris-HCl pH 6.8, and bromophenol blue was added to each sample to the following final concentrations: 2% SDS, 10% glycerol, 50 mM Tris-HCl, and 0.02% bromophenol blue

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Valenciano et al., 2012)

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Doa1 targets ubiquitinated substrates for mitochondria-associated degradation

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