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Immunohistochemical staining Immunohistochemical staining was carried out on representative formalin-fixed, paraffin-embedded tissues using the Bond Polymer Refine Detection system (Leica Biosystems Newcastle Ltd., Newcastle, UK) according to the manufacturer's instructions, with minor modifications

It is also worth noting that APAP is used in combination with other drugs, particularly opioids and diphenhydramine, without public awareness (Rajaram and Subramanian, 2018)