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The dismutation of superoxide can also occur spontaneously, but such reaction is 10 4 times slower than the enzymatic dismutation by SOD2

Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

Aging: Natural levels of glutathione decline with age, making older adults more susceptible

lactamica carriage is highly prevalent in young children peaking around age two and decreases with age (Gold et al., 1978