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Herein, murine melanoma cells B16F10 (ATCC, Manassas, VA, USA) culture was maintained in Dulbecco's Modified Eagle Medium (DMEM) (Welgene, Gyeongsan, Gyeongbuk, Korea) containing 10% fetal bovine serum (FBS) (Welgene, Gyeongsan, Gyeongbuk, Korea), and penicillin (100 U/mL)/streptomycin (100 g/mL) (Welgene, Gyeongsan, Gyeongbuk, Korea) at 37 C under 5% CO 2 atmosphere in a CO 2 cell incubator (NU-4750G, NuAire, Plymouth, MN, USA)

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Singh suggests avoiding sitting for prolonged periods