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Seven of the nine pathways were involved in amino acid metabolism, and another was peroxisome proliferator-activated receptor (PPAR) signaling, which is a potent regulator of cellular metabolism

Bowel incontinence (inability to control the bowel) is a common problem

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Co-localization was also performed due to the manufacturers protocols using Puromycin (Sigma, St Louis, MO) as a negative control and anti-HNE (ab48506) (Abcam, Cambridge, GB) (1:50) and anti-Pus7 (PA5-54983) (Thermo Fisher Scientific, Waltham, MA) (1:200)