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Distilled or deionized water Precision pipettes to deliver 2 ul to 1 ul volumes Adjustable 1-25 ul pipettes for reagent preparation 100 ul and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Prepare all reagents, samples and standards as instructed in the manual

In HG-induced rat cardiomyocytes (H9C2) and HFD/STZ-induced C57BL/6J mouse models, it was found that mitophagy was impaired under hyperglycemic conditions, and that canagliflozin was able to increase the phosphorylation of AMPK and activate PINK1/Parkin-dependent mitophagy at the same time, which could improve the function of mitochondria and play a certain role in the DCM therapeutic effects [177]

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