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Nonetheless, this represents an effort to capture benefits that are not easily quantifiable (see, for example, Box 4-1 and Box 4-2, which outline two early successes from the STTP process at AF)

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Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)