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We performed single molecule fluorescent i n s itu hybridization (smFISH) on dissected gonads with lag-1 mRNA complementary probes, analyzing expression quantitatively by counting foci along the distal-proximal axis of the germline [43] ( Materials and Methods )

A phylogenetic analysis based on full-length protein sequences revealed that the SmGSTs can be arranged into 2 main clades: Clade I and Clade II (Figure 1A)

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