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Bivariate analysis was conducted to identify the correlation between variables: data with normal distribution were analyzed using Pearson test, and data with non-normal distribution were analyzed using Spearman test

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Interaction between microbiota and immunity in health and disease

Immunoprecipitation For immunoprecipitation assays, NIH-3T3 cell or tissue protein, respectively, was extracted with a non-denaturing cell or tissue extraction buffer (Invitrogen/ThermoFisher, Carlsbad, CA)