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Per the manufacturers protocol, slides were incubated with the kit amplifier/enhancer for 20 min at room temperature (25C), followed by incubation with the HRP-conjugated secondary reagent for 30 min at room temperature
Glycyrrhizin , the main extract from licorice ( Glycyrrhiza glabra ), is a natural antioxidant, anti-inflammatory, antifibrotic and antiviral agent widely used in the treatment of chronic hepatitis 511
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Nonetheless, during methicillin-resistant Staphylococcus aureus (MRSA) skin infection, rescuing PGE 2 effects in diabetic mice, using a topical treatment with misoprostol, rescued DC migration to draining lymph nodes, Th17 differentiation, and improved host defense [51] suggesting that PGE 2 effects during efferocytosis of infected cells are context-dependent
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