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Companion satellite-cell cultures, prepared at the same time, were immunostained at 30-h post-plating with D3 anti-desmin mAb and DAB (a chromogenic HRP-substrate that forms an insoluble brown reaction product) to determine the percentage of myogenic cells present 18 ,19 cultures with less than 95% DAB-positive cells (namely, less than 95% desmin-positive myogenic purity) were not used for experiments just to ensure the reliability of data specific to myogenic cells in the employed primary cell culture systems

The wound area was photographed immediately after wounding (0 hours), and again at 12 hours postscratch using brightfield exposure at 10 magnification on a Nikon eclipse 80i epifluorescence microscope

Claims surrounding BPC-157 benefits derive exclusively from preclinical animal studies, which regulatory bodies do not accept as sufficient evidence for human therapeutic approval

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