24 The ribs were digested by pronase and collagenase D and chondrocytes were plated onto a 100 mm Petri dish with DMEM (Gibco, Invitrogen) supplemented with 1% penicillin/streptomycin sulfate (Gibco, Invitrogen), 1% L -glutamine (Gibco, Invitrogen) and 10% fetal bovine serum (Gibco, Invitrogen) at 37 C in a 5% CO 2 humidified incubator
On the other hand, in a study in conscious dogs during double blockade of the RAS (combined ACE inhibition and aldosterone receptor blockade), Ang III was significantly more potent than Ang II in increasing aldosterone release 55, 109 and also produced a very potent antinatriuretic effect already at a dose not producing an increase in blood pressure
*Correspondence: Yufei Liu, [email protected] Xu, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
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