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The biological effects downstream of cAMP elevation have been previously demonstrated to be predominately mediated by cAMP-dependent protein kinase (PKA), which phosphorylates cAMP-response element (CRE) binding protein (CREB) (21)

Ensure needle depth is sufficient (0.5 to 1.5 millimeters) and that the peptide solution is applied immediately after needling while channels remain open

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