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Evolution of the calcium-based intracellular signaling system

For detection of SNIP1 (Thermo Scientific, PA5-29412, 1:1000) and BUD13 (Bethyl, A303-321A, 1:2000) proteins by western blotting, the SF3B1 pull down protocol was modified in a way that six in-vitro kinase reactions were pooled together for each condition (6 times with and 6x without flag-tagged CDK11 kinase)