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Liu S, Liu F, Jia H, Yan Y, Wang H, Guo X

QSSA was used in this iteration of the design to assume enzyme levels stayed constant, but further investigation of the induction of oxidative stress genes by ROS and how this might change enzyme concentrations would be of value

Immunoblotting of HO-1 and IL-6 After boiling liver homogenates (50 g of total proteins) for five minutes in a loading buffer containing 2-mercaptoethanol, the samples were run on a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for 2 h at 100 V

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