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Next, the cells were incubated in DNA labeling solution for 60 min at 37 C, followed by sequential 30 min incubations of BrdU antibody solution and 7-AAD at RT

PI3K/AKT/mTOR: the regulator of anabolism Among the three pathways, the PI3K/AKT/mTOR pathway is arguably the pathway most directly linked to metabolic reprogramming, as it coordinates glucose uptake, amino acid transport, protein synthesis, and lipid metabolism

Feeding Saccharomyces cerevisiae fermentation postbiotic products alters immune function and the lung transcriptome of preweaning calves with an experimental viral-bacterial coinfection