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Three main alterations take place during acute inflammation: increased capillary permeability, which permits larger serum molecules to enter the tissues, increased leukocyte migration into the tissue, and increased blood flow to the affected area (Al-Kofahi et al

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J Pharmacol Experimen Ther Nov

Plates were incubated at room temperature for 30-60 min in the dark, and fluorescence was read at Ex/Em = 490/520 nm