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The release of neurotransmitters, specifically glutamate, into the extracellular compartment results in an overstimulation of glutamate receptors linked to ion channels and creates prolonged membrane depolarization, depletion of ATP stores, and an increase in intracellular calcium derived from both extracellular and intracellular stores, indicating disturbances in mitochondrial function

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2-D electrophoresis was performed to elucidate the reasons for this discrepancy, and the results indicated obvious changes in central metabolism and amino acid metabolism in the penta-mutant