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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )
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[ 13 C 4 , 15 N 2 ]-GSSG was prepared from the oxidation of [ 13 C 2 , 15 N]-GSH as described 96
