All cells except for hNF-1 were grown and maintained as monolayer cultures at 37 C in 5% CO 2 in a humidified atmosphere, using DMEM (high glucose with l-glutamine and phenol red, Fujifilm Wako Pure chemical corporation), supplemented with penicillinstreptomycin solution (100) (Fujifilm Wako Pure Chemical Corporation), and 10 vol% FBS (Sigma-Aldrich Co., Llc.)
Its not a problem, its just weird indeed I have a new appointment next week to get my full arm done (shading, background etc), so Im gonna sit in the tattoo chair for about 8hrs
Bao M, Lou Y (2006) Flavonoids from seabuckthorn protect endothelial cells (EA.hy926) from oxidized low-density lipoprotein induced injuries via regulation of LOX-1 and eNOS expression
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