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In the short term, inflammation is beneficial because it helps protect damaged tissues and initiate healing

Every four weeks, the dose is gradually escalated (to 0.5 mg, 1.0 mg, 1.7 mg, and finally the maintenance dose of 2.4 mg per week) until the patient reaches their target clinical maintenance level

doi: 10.3390/ijms20040941 246 MotoriE.PuyalJ.ToniN.GhanemA.AngeloniC.MalagutiM.et al

Afterwards, samples were washed once with 0.1% Triton X-100/PBS, twice with PBS, and subsequently, cells were quenched in 100 mM glycerol/PBS for 10 minutes