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doi: 10.1016/j.cryobiol.2023.02.003 24 KutluyerF.KayimM.retmenF.BykleblebiciS.TuncerP

Furthermore, potential crosstalk with other metabolic hormones could be a significant factor

Reaction mixture II for each sample was collected as follows: 1160 l of 0.1-M Tris-HCl buffer with 0.34-mM EDTA (pH 8.5) were sequentially added to 108 l of each supernatant containing residual GSH, and then at the same intervals, 9 l of a solution of 5,5-dithiobis(2-nitrobenzoic acid) or Ellmans reagent (4 mg/ml of absolute methanol) was added to each sample (in the same sequence of samples)

Your neurons can maintain peak function for hours without the glucose crash, and your muscles get steady fuel that doesn't tap into glycogen stores