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For quantitative TMAO analysis, plasma specimens were diluted 1:10 in acetonitrile containing a spiked isotopic standard, TMAO-D9 (Cambridge isotope DLM-4779-1)

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The presence of three cationic lysine (Lys) residues, one anionic glutamic acid (Glu), and a tryptophan (Trp) in SmAP121 likely enhances its membrane-binding capacity

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