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Deryabin et al., 2020)

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Since this regimen restricts methionine in the absence of cystine, we restricted both methionine and cystine in our cell culture DMEM medium by combining methionine/cystine-free DMEM with 10% dialyzed FBS, which resulted in a restricted DMEM medium (MCR) containing 0.12 M methionine and undetectable levels of cystine

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