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Step 4 Transfer slowly Insert the needle into the peptide vial at an angle

Key considerations include: clear inclusion criteria (e.g

PCA is based on the quantitation of metabolites peaks after normalization to total sum and pareto scaling showing clustering of sample cohorts with no overlap of the ellipses (ellipses represent 95% confidence intervals), displaying good separation between and repeatability within cohorts of samples

Hum Genomics 18:129 Kuhn M, Kyei NNA, Cramer B et al (2025) Online solid phase extraction-LC-MS/MS with two step peak focusing for sensitive multi-analyte analysis of mycotoxins in urine