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Depletion of Gln in vitro can lead to an impaired proliferation and dysfunction of T-lymphocytes 16,17,18

It has shown a pronounced anti-inflammatory effect, leading to decreased levels of various proinflammatory cytokines, decreased oxidative stress, as well as increased levels of adiponectin, which exerts an anti-inflammatory effect, as well as increases insulin sensitivity [66]

The serum insulin and glucagon levels were determined using a commercial Rat/Mouse Insulin enzyme-linked immunosorbent assay (ELISA) kit Merck Millipore (Kenilworth, NJ, USA) and by competitive inhibition enzyme immunoassay technique for quantitative measurement, using the enzyme-linked immunosorbent assay kit from Cloud-Clone Corp