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Following triple staining (GPX1 insulin + glucagon), the overall cellular intensity for GPX1 (brown) and its distribution pattern in all islets with more than 20 estimated cells were graded visually following display of acquired images by Photoshop with sufficient magnification for ease of determining the distribution of cells as follows

Eschenbrenner, M., Chlumsky, L

Some researchers might push this to 6 weeks, but we recommend exercising extreme caution beyond the 4-week mark to ensure optimal efficacy

As the immune effects of aCD3 are well established, our analyses focused on the pancreatic islet compartment, using spatial transcriptomics and immunostaining to examine how GLP1-E2 remodels beta cell (transcriptional) states