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Conventional medicine considers arginine, cysteine, glycine, glutamine, proline, and tyrosine as conditional amino acids that may not be produced when a person has catabolic issues , for instance, or in premature babies
Western blotting Cell or tissue samples were lysed with RIPA buffer containing 1% protease inhibitor, and the protein concentration of cell lysates was measured by BCA protein assay kit (Beyotime, China)
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