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Make sure you are drinking plenty of water and replenishing electrolytes especially if you are experiencing any of these symptoms

Cover with the Plate Cover and incubate for 80 minutes at 37C

In order to monitor the intracellular translocation of agonists, as well as GLP-1Rs, we used fluorescently labeled exendin-4, exendin-phe1, and exendin-asp3 conjugates, with fluorescein isothiocyanate (FITC) at position K12, which we previously found useful for labeling GLP-1R 28