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Overall, mast cells have 50 200 granules in the cytoplasm of the cell, storing histamine, heparin, tryptase, cytokines, etc.[ref] The image below shows mast cell (stained purple) degranulation under a microscope.[ref] Allergies: Activating mast cells by Type 1 hypersensitivity In addition to reacting to antigens on pathogens, such as bacteria, the IgE can also be primed to link with antigens on proteins that are not pathogens, referred to as allergens

The industry standard for this is Bacteriostatic Water, which contains a small amount of benzyl alcohol to prevent bacterial growth, ensuring the solution remains sterile through multiple uses

Wan J , Forsyth AM , Stone HA