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Elevated levels of NNMT activity is associated with reduced cellular energy expenditure and increased fat storage

Immunoproteasome activity 20 g of liver samples from ibuprofen-treated and control animals were incubated with immunoproteasome buffer (50 mM Tris, 5 mM MgCl 2 , 20 mM potassium chloride (KCl), 1 mM DTT (freshly added), pH 7.5), and inhibitor or an equal volume of dimethyl sulfoxide (DMSO) for 20 min at room temperature

Funding This work was funded by the National Natural Science Foundation of China (Grant No

Starting with a lower dose and slowly increasing over several weeks helps most people manage these effects