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Separation of allicin from extract was performed using a Spherisorb ODS2 column (4.6x250 mm, 5 m, waters, Ireland) and methanol (75%) and water (phosphate buffer pH = 3), as a mobile phase, with a flow rate of 1 mL min -1 , detecting allicin at the wavelength of 254 nm

Aldehyde dehydrogenase-2 (ALDH2) opposes hepatocellular carcinoma progression by regulating AMP-activated protein kinase signaling in mice