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This mechanism distinguishes Dihexa from conventional nootropics that modulate neurotransmitter systems without promoting structural brain changes

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Briefly, cells were cultured in DMEM/F12 supplemented with 10% FBS, 100 U/ml penicillin, 100 mg/ml streptomycin, 8 g/ml biotin, and 4 g/ml pantothenic acid (all from Thermo Fisher Scientific Waltham, MA, United States)

Furthermore, in the M group, the health status of the ovarian cells significantly worsened, as indicated by a decreased proliferating cell nuclear androgen (PCNA) signal and an increased TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling) signal (Fig