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A BMI of 25 or higher around the index date Prior engagement (one or more outpatient encounters more than one year prior to index overdose AND one or more outpatient encounters after index overdose) Study population: exclusion Patients with: An assault-related overdose (ICD-10-CM code T* with 7th character = 3) A GLP-1 order prior to their first overdose encounter A methadone overdose (ICD-10-CM code T40.3X*) diagnosis Index date First qualifying overdose encounter (ED or inpatient) which must occur within the study period

AD model mice suggested an increase in Hdac1/Hdac2, glycolysis/H4K12la/PKM2 signaling, and a decrease in Jmjd3, a histone H3K27me3 demethylase (Datta et al., 2018

The bioavailability of nutrients largely depends on the health status of the individual

Inflammation Something called gut permeability, or how much fluid and other nutrients make their way through the intestinal wall, increases as you go through menopause