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Originally termed Stein-Leventhal syndrome, PCOS represents a multisystem disorder characterized by intricate associations with metabolic dysregulation and hypothalamic-pituitary-ovarian axis (HPOA) dysfunction, posing substantial threats to fertility during womens prime reproductive years ( PCOS typically manifests during adolescence and is diagnostically characterized by three main features: namely long-term ovulatory dysfunction, hyperandrogenism (HA), and polycystic ovarian morphology, which form the basis of the diagnostic criteria for PCOS (Siddiqui et al., 2022)

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Analyses of cross-sectional data from 13,887 US adults included in the Third National Health and Nutrition Examination Survey (NHANES III, 1988-1994) failed to show any significant associations between serum selenium concentrations and mortality from CVD, coronary artery/heart disease (CAD), or stroke (91)

Quantitative real-time PCR Total RNA was isolated with Trizol from tumor as well as normal tissue samples collected from each patient and then purified using the RNeasy Mini Kit (Qiagen) according to the manufacturers protocol