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Arabidopsis maintains telomere length homeostasis in the face of heat shock, drought and high temperature ( Caenorhabditis elegans which are grossly extended in space ( Telomere length is primarily maintained by telomerase, a ribonucleoprotein complex containing a reverse transcriptase TERT and an integral RNA template TR

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The CRISPRi-dCpf1 system uses inactivated Cpf1 (dCpf1), which does not generate DSBsthis avoids the lethal issue caused by the low homologous recombination efficiency of Clostridia and achieved 3- to 100-fold gene silencing efficiency in 27 Clostridium strains